Annals of Tropical Medicine and Public Health
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ORIGINAL ARTICLE
Year : 2013  |  Volume : 6  |  Issue : 4  |  Page : 441-445

Molecular detection of BRO β-lactamase gene of Moraxella catarrhalis isolated from Sudanese patients


1 College of Medicine, Al-jouf University, Kingdom of Saudi Arabia, Al-Jouf, Kingdom of Saudi Arabia
2 Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Medina, Kingdom of Saudi Arabia; Department Clinical Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan
3 Department of Microbiology, College of Medical Laboratory Science, El neelain University, Khartoum, Sudan

Correspondence Address:
Mogahid M El Hassan
Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology P. O Box 407, Khartoum, Sudan

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1755-6783.127796

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Objective: To determine the frequency of BRO β-lactamase gene among Moraxella catarrhalis strains isolated from individuals with different clinical manifestations in Sudan. Design: Prospective cross-sectional laboratory-based study. Setting: Different hospitals and health centers in Khartoum State, Sudan. Materials and Methods: Fifteen (n = 15) β lactamase positive M. catarrhalis isolates, isolated from Sudanese patients with different clinical pictures were examined for the presence of BRO β-lactamase gene using restriction fragment length polymorphism (RFLP) technique. Results: Out of 400 patients, M. catarrhalis was isolated from 15 (3.75%) patients. All of the M. catarrhalis isolates were confirmed as β-lactamase producer, from which 11 isolates (73.3%) were positive for a BRO-1 gene using RFLP, whereas 4 isolates (23.7%) were BRO negative. Conclusion: The majority of β-lactamase gene of Moraxella catarrhalis in Sudan is BRO-1 gene. No BRO-2 gene was detected in this study; hence, the resistant phenomenon of Sudanese isolates of M. catarrhalis may attribute to BRO-1 gene.


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