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Table of Contents   
LETTER TO THE EDITOR  
Year : 2015  |  Volume : 8  |  Issue : 4  |  Page : 138
Primer design and in silico PCR test for Ebola virus detection


1 Department of Medical Center, Sanitation 1 Medical Academic Center, Bangkok, Thailand
2 Department of Tropical Medicine, Hainan Medical University, Hainan, China; University of Nis, Nis, Serbia; Joseph Ayobabalola University, Nigeria; Dr. DY Patil Medical University, India; Surin Rajabhat University, Surin, Thailand

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Date of Web Publication7-Aug-2015
 

How to cite this article:
Joob B, Wiwanitkit V. Primer design and in silico PCR test for Ebola virus detection. Ann Trop Med Public Health 2015;8:138

How to cite this URL:
Joob B, Wiwanitkit V. Primer design and in silico PCR test for Ebola virus detection. Ann Trop Med Public Health [serial online] 2015 [cited 2020 Mar 29];8:138. Available from: http://www.atmph.org/text.asp?2015/8/4/138/162395
Dear Sir,

Ebola virus infection is a public health consideration worldwide. This infection manifests as a serious viral hemorrhagic fever with high mortality. To diagnose the infection requires the definitive molecular diagnosis. [1] Setting a molecular diagnostic tool is the basic requirement for any setting, to prepare for the possible emergence of disease. Here, the authors report an attempt to set the molecular diagnostic tool for Ebola virus infection. In this short article, primer design and in silico polymerase chain reaction (PCR) test for Ebola virus detection are reported on. The primary sequence of Ebola virus, Zaire ebolavirus isolate Ebola virus (NC_002549.1), was used as the template. The standard tool, Primer 3: WWW Primer, was used for primer design, and the result is shown in [Figure 1]. The derived primers were further tested for PCR. The in silico PCR test was performed. According to the test, the designed primer is acceptable for PCR testing and there is no cross-reaction. This is comparative to the PCR tool that is developed from the patients' samples. [2]
Figure 1: Designed primers

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   References Top

1.
Grolla A, Lucht A, Dick D, Strong JE, Feldmann H. Laboratory diagnosis of Ebola and Marburg hemorrhagic fever. Bull Soc Pathol Exot 2005;98:205-9.  Back to cited text no. 1
    
2.
Leroy EM, Baize S, Lu CY, McCormick JB, Georges AJ, Georges-Courbot MC, et al. Diagnosis of Ebola haemorrhagic fever by RT- PCR in an epidemic setting. J Med Virol 2000;60: 463-7.  Back to cited text no. 2
    

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Correspondence Address:
Beuy Joob
Department of Medical Center, Sanitation 1 Medical Academic Center, Bangkok, Thailand

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1755-6783.162395

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