Annals of Tropical Medicine and Public Health
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ORIGINAL ARTICLE
Year : 2013  |  Volume : 6  |  Issue : 6  |  Page : 653-657

LRSA suspect, taken to molecular court, PCR trialled, proven guilty


1 Department of Microbiology, Calcutta Medical College, Kolkata, West Bengal, India
2 Department of Microbiology, Calcutta National Medical College, Kolkata, West Bengal, India

Correspondence Address:
Dibyendu Banerjee
27-H, Rajkrishna Street, Uttarpara - 712 258, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1755-6783.140243

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Background: A 31-year-old patient was admitted in our Medical College and Hospital following a road traffic accident, and with a history of Linezolid treatment for 10 days. Materials and Methods: Culture from wound swab revealed multiple pathogens. Among them, Staphylococcus aureus turned out to be Methicillin resistant (MRSA), and also showed resistance to Linezolid disc diffusion. Then PCR of the resistant strain as well as a sensitive strain followed by DNA sequencing was done. Results: Sequencing of the domain V region of the 23S rRNA gene revealed the presence of a G2576U mutation in LRSA (LZD-resistant S. aureus) in two 23s rRNA copy, rrn1 and rrn5. Conclusions: The patient's wound was infected with an MRSA strain, which was LRSA as well. LRSA is a rare occurrence, and rarely reported from Indian subcontinent. A constant vigil is necessary to detect this resistance, if possible with tools of molecular epidemiology.


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