|Background: The occupational injury can lead to partial disability or sick leave of nursing staff and prehospital emergency services for a certain period of time. This study aimed to compare the frequency of occupational hazards emergency medical and nursing staff of Intensive Care Units (ICUs) in Hamadan. Materials and Methods: In this cross-sectional study, a standard questionnaire was distributed to all emergency personnel, doctors, and nurses of ICU in Hamadan. The collected data analyzed by SPSS version 16 to compare occupational injuries that inferential statistics, Chi-square test, and Fisher’s exact test were used. Results: Out of 340 questionnaires distributed among the emergency medicine personnel and nurses, 147 questionnaires were returned. The most frequent occupational injuries (84.5%) by aged 20–30 years, Also the highest frequency of injury in the area of emergency medicine personnel spine (66%) and nurses’ legs (72.3%). Its difference between the areas affected by the type of job and the relationship between job and the risk factors causing significant damage was compared between nurses and emergency medicine personnel (P > 0.001). Conclusion: To prevent damage to the occupational and medical personnel because of the sensitivity of musculoskeletal injuries, recommended strategies for the prevention of occupational injuries to be included in training programs for health personnel.