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Table of Contents   
LETTER TO THE EDITOR  
Year : 2017  |  Volume : 10  |  Issue : 1  |  Page : 260-261
Cloning of new third generation lentiviral gene expression vector: A preliminary report


1 Sanitation Medical Academic Center, Bangkok, Thailand
2 Hainan Medical University, Haikou, Hainan, China

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Date of Web Publication5-May-2017
 

How to cite this article:
Joob B, Wiwanitkit V. Cloning of new third generation lentiviral gene expression vector: A preliminary report. Ann Trop Med Public Health 2017;10:260-1

How to cite this URL:
Joob B, Wiwanitkit V. Cloning of new third generation lentiviral gene expression vector: A preliminary report. Ann Trop Med Public Health [serial online] 2017 [cited 2019 Dec 7];10:260-1. Available from: http://www.atmph.org/text.asp?2017/10/1/260/205555
Dear Sir,

Molecular cloning is the advanced biomedical technology at present. The technique can be very helpful in tropical medicine. It can serve several purposes such as the finding for drug resistance gene,[1] finding for epitope,[2] and the construction of gene expression vector. Here, the author proposes for an example of successful performing on cloning of new third generation lentiviral gene expression vector. The VectorBuilder technique was used and the derived vector is, namely, “plentivirus (LV) Expression [Exp]-Neo-cytomegalovirus (CMV) > enhanced green fluorescent protein (EGFP)” [Figure 1], size 8828 bp. This is a lentiviral gene expression vector (third generation). The inserted promoter is “CMV”, inserted open reading frame (ORF) is “EGFP” and inserted marker is “Neo”. The sequence of the derived vector is shown in [Figure 2]. Further, restriction analysis, using primers as M13 (−48) reverse primer 8729-8752 and T7 primer 5971-5989 showed the result as in [Figure 3]. Of interest, the new cloned lentiviral gene expression vector has a CMV promoter, which is previously reported for possible usefulness in human immunodeficiency virus (HIV) medicine.[3] The application of the new derived vector has to be further studied.
Figure 1: Derived vector

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Figure 2: Sequence of derived vector

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Figure 3: PCR product according to the mentioned primers in restriction analysis

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Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

1.
Clos J, Choudhury K. Functional cloning as a means to identify Leishmania genes involved in drug resistance. Mini Rev Med Chem 2006;6:123-9.  Back to cited text no. 1
[PUBMED]    
2.
Miles MA, Wallace GR. Cloning of microbial epitopes relevant for T- and B-cells. Behring Inst Mitt 1991:133-41.  Back to cited text no. 2
[PUBMED]    
3.
Konopka K, Lee NS, Rossi J, Düzgüneş N. Rev-binding aptamer and CMV promoter act as decoys to inhibit HIV replication. Gene 2000;255:235-44.  Back to cited text no. 3
    

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Correspondence Address:
Dr. Beuy Joob
Medical Academic Center, Bangkok
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1755-6783.205555

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    Figures

  [Figure 1], [Figure 2], [Figure 3]



 

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