Annals of Tropical Medicine and Public Health
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Year : 2018  |  Volume : 11  |  Issue : 3  |  Page : 83-86

Phytochemical screening and thin-layer chromatographic analysis of tannin from Dryopteris, Asplenium, and Davalliain, Tahura, Batu, Indonesia

Department of Biology, Faculty of Matemathic and Natural Sciences, Malang State University, Jalan Semarang 5-Malang 65145, Indonesia

Correspondence Address:
Dr. Murni Sapta Sari
Deparment of Biology, Faculty of Matemathic and Natural Sciences, Malang State University, Jalan Semarang 5, Malang 65145
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ATMPH.ATMPH_36_17

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Context: Forest area in Tahura R. Soerjo has high moisture level with rainfall 1488 mm3/year, so it provides an optimum condition for ferns growth. Tannin is one of the secondary metabolites that have many benefits; it can be found in various amounts in plant body parts. Aim: The study aims to identify the tannin in three fern genera, namely, Dryopteris, Asplenium, and Davallia that were obtained from Tahura R. Soerjo. Materials and Methods: Both epiphytic (Davallia) and terrestrial (Asplenium and Dryopteris) fern samples were obtained from Taman Hutan Raya Raden Soerjo, Cangar, Batu city in May– August 2015. Powdered fern samples were extracted through maceration method using methanol (1:10). The methanol extract of fern were tested for the tannin presences using phytochemical screening and layer chromatography. Results: Thin-layer chromatography was done to confirm the screening results that indicate the existence of tannin. Phytochemical screening for gallotannin in methanol extracts positive results. All extracts perform a different number of spot and Rf value.Dryopteris and Davallia frond showed reddish-orange spot on the plate, while Dryopteris rhizome,Davallia rhizome,Asplenium frond, and Asplenium rhizome showed greenish brown on the plate.Conclusion: The phytochemical screening including tannin confirmation and thin-layer chromatography showed that all part (frond and rhizome) from Dryopteris,Davallia, and Asplenium contained gallotannin and tannin.

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