A recent study found that adding the NS1 antigen to the IgM/IgG test for the diagnosis of dengue is more accurate than using the test alone. The researchers conducted a retrospective study of patients who had been infected with dengue in the United States and Vietnam and compared the results with those obtained from a control group. The results showed that the combination was more accurate than either one individually.
The new ELISA method was also found to be more sensitive than the traditional methods of IgM/IgG testing. The study included a total of 261 patients who were diagnosed with dengue. The sensitivity of the NS1 antigen test was greater than the other two tests. The NS1 antigen is also used to determine whether a patient has the disease. The NS1 antigen antibody ELISA is available at the University of Pennsylvania Medical Center and many other medical centers in the United States and the Philippines.
This study used a comparison of the NS1 antigen test and the IgM/IgG test to see which was more accurate. The IgM/IgG tests had higher DOR and PPV, but were less accurate in LR- and NPV. The IgM/IgG test was significantly more sensitive but failed to significantly improve the accuracy of dengue diagnosis.
The results of this study showed that adding the NS1 antigen test to the IgM/IgG test significantly increased the probability of detecting dengue infection. While the NS1 antigen alone has not been able to catch every case, it did double the sensitivity of the IgM/IgG test. The SD IgM/IgG RDT was more sensitive than the SD IgM RDT.
The SD NS1/IgM RDT was more sensitive than the IgG RDT. Both tests had high sensitivity, which meant that they had similar results. In addition, the SD NS1/IgG RDT had a lower sensitivity, but it performed better than the serology-only tests. The NS1/IgG RDT performed better than the SD IgM/IgG test.
The NS1 antigen test has a moderate sensitivity compared with the SD NS1 ELISA. Despite being a more specific NS1 antigen test, it was not significantly more accurate than the SD NS1 ELISA. The ViroTrack Dengue Acute sensitivity was higher than the SD NL-IgM test. The SD NS1 ELISA had a better sensitivity compared to the ViroTrack Dengue RDT.
Although the SD Bioline Dengue Duo underperformed the SD NS1 and IgM individually, their combined performance was superior when they were used as a single test. Both components improved sensitivity, NPV, LR-, while the SD NS1 test was less sensitive than the IgM-based RDT. Additionally, the combination improved diagnostic accuracy and sensitivity.