Background: Chronic renal failure (CRF) is one of the most prevalent diseases of human societies, especially in Iran. Despite all advances that have been made so far, hemodialysis with all its complications is being applied as the novel treatment strategy for such individuals. Objective: The aim of this study is to investigate the effect of hemodialysis on the change rate of serum L-creatinine and lipid peroxidation levels. Materials and Methods: This study is an analytical research which used a convenience sampling method. Malondialdehyde (MDA) as the indicator of lipid peroxidation and L-creatinine was measured in 27 patients (17 males and 10 females). Malondialdehyde was calculated using the standard thiobarbituric acid (TBA) method and L-creatinine through the t-test enzymatic method (Roche kit) (SpectronicGenesys 340 nm). Results: The mean and standard deviation of serum L-creatinine before and after dialysis was 7.67 ± 3.6 mg/L and 2.07 ± 1.6 mg/L, respectively (P < 0.001). The MDA mean of serum before and afterdialysis was 4.17 ± 1.24 mol/L and 4.98 ± 1.2 mol/L, respectively (P < 0.001). Conclusion: Analyses show that serum L-creatinine reduces considerably after dialysis. In addition, the results suggest that stress oxidative reduces following hemodialysis in these patients, which is observed in the form of the increased lipid peroxidation.